Journal Title: تابستان 96 Article title: The Effects of Adipose-Derived Stem Cells (ADSC) Autograft in the Viability of Random Skin Flap in Rats
Article PDF File: Page From: 45 To: 53
Article abstract: Introduction & Objective: Flap necrosis caused by inadequate blood flow to a common complication of surgery is reconstructive. Since the angiogenic potential of stem cells has been shown, the purpose of this study was to evaluate the effect of adipose-derived stem cells autograft rate of random skin flap angiogenesis animal model rat.
Materials & Methods: This experimental study in 1394 in laboratory animals Hazrat Fatima (SA) was conducted. In this study, 24 male Sprague-Dawley, rats weighing 300-350 g were divided into two groups after anesthesia. In the study group of stem cells from fat tissue autograft were inguinal area and cultured and then injected subcutaneously at the base of random flap in the control group received saline. On days 7 and 14 photographs were taken to determine the extent of necrosis flap and then the flap base for histopathological examination on day fourteen and counting the number of capillaries were sampled. At the end, the data were analyzed by using 19 SPSS software and paired samples t-test.
Results: The comparison between the two groups showed areas of flap survival on the seventh day of the stem cell group 10.592 ± 1.095 and in the control group 5.793 ± 2.023 and on the fourteenth day respectively 10.769 ± 0.934 and 5.519 ± 2.107, the difference was significant (P < 0.001). Results angiogenesis stem cell group 27.666 ± 10.201 and control 12.750 ± 6.810 and the difference in the two groups was significant (P < 0.001), so that the average of the group is more stem cells.
Conclusions: These findings suggest that adipose-derived stem cells have the potential to improve the survival of random flap. This mechanism can also differentiate these cells into endothelial cells or indirectly by increasing angiogenesis is.
Article KeyWords: Adipose-Derived Stem Cells, Angiogenesis, Random Flap