Journal Title: زمستان 1402
Article title: Construction and Characterization of Polyphenylene Graphene / PVDF Self-Stimulated Neural Conduction Channels for the Growth and Proliferation of PC12-Like Neuronal Cells

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Article abstract:
Introduction & Objective: So far, various studies have been carried out in order to design different methods for the construction of the nerve channel with the aim of repairing the nerve tissue. The purpose of this study is nerve repair surgery by making a nerve conductive channel with the aim of self-stimulation. In this study, polycaprolactone (PCL) and polyvinylidene fluoride (PVDF) channel, in combination with polyaniline/graphene nanoparticles (Polyaniline Graphene-PAG) and gelatin, was prepared by a two-way and simultaneous electrospinning approach. Materials & Methods: At first, solutions containing 13% by volume of polycaprolactone and 19% by volume of polyvinylidene fluoride in the common solvent of dimethylformamide (Dimethylformamide - DMF) and acetone were dissolved separately, and then both solutions were mixed together. became In the next step, 28 % by weight and volume of gelatin solution was dissolved in distilled water and acetic acid, and polyaniline/graphene nanoparticles with 0, 1, 2, and 3 percent by weight and volume were homogeneously mixed in the gelatin solution, and then two-way and simultaneous electrospinning process Was performed. Scanning Electron Microscope (SEM) was used to examine the morphology of the fibers. In order to determine the optimal amount of polyaniline / graphene nanoparticles for nerve conduction, electrical conductivity and piezoelectric properties of the fabricated scaffolds were investigated and also the survival percentage of pheocrocytoma cell line (PC12) on the scaffold was calculated. Data were calculated as mean ± standard deviation (Mean ± SD). The significant level of differences was determined to be (P < 0.05). Results: The results was showed that with increasing the percentage of polyaniline/graphene nanoparticles up to 2% by weight, the amount of electrical conductivity and output voltage increased, but at a concentration of 3% by weight, the amount of electrical conductivity and also the output voltage decreased. Scanning electron microscope observations showed that the average diameter of nanofibers decreased up to a concentration of 2% by weight, but at a concentration of 3% by weight of polyaniline/graphene nanoparticles, the diameter of nanofibers increased. Also, the biodegradation of electrospun scaffolds showed that the increase of nanoparticles caused a decrease in degradability. Conclusions: In nerve repair surgery, the presence of polycaprolactone and gelatin along with polyaniline/graphene nanoparticle increased the nerve conduction and also the use of polyvinylidene fluoride in the electrospun scaffold will improve the self-stimulation property in the nerve conduction channel, which can be a suitable substrate for The growth and cell proliferation of the nerve tissue, and hence the probability of success in the surgery increases to its maximum extent.
Article KeyWords:
Neural Conduction Channel, Self-Excitation Property, Nanoparticles, Mechanical Properties
Article Authors:

	دکتر حمیده جاویدی	First Author
	دکتر مهدی موحدی	Other Author	dr.mehdimovahedi@gmail.com