Journal Title: زمستان 89
Article title: Comparison between the Effect of Amniotic Membrane and Vein Conduit in Nerve Repair - Animal Study

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Article abstract:
Introduction & Objective: Annually, a lot of patients experience motor problems due to nerve lesions. Reviving the muscle activity is essential to bodily function. There are a variety of methods which can be adopted to repair motor difficulties. The aim of this study was to compare and contrast the three methods of invasive transplantation surgery, namely: end-to-end alone, end-to-end coverage with vein tube and coverage with amniotic membrane. Materials & Methods: 36 male rats weighing 250-350 grams, and of the same breed were randomly divided into three equal groups. After being treated with anesthesia, the sciatic nerve in all three examined rat groups was cut. Surgery was performed on the three groups of rats, using the three mentioned invasive methods. The first group of rats were operated upon using two suture ends, the second group was operated upon using the amniotic membrane, and for the third group, the venous conduit was used. Twelfth weeks after surgery, EMG was used to determine the restoration of nerve function. For each rat axon counted. Furthermore, the Foot Print test was used in the first and the Twelfth weeks to determine nerve function. Paired sample t-test was used to compare the results of the first and twelfth weeks, and one way Anova test used to compare the results of the three groups. Results: The results of this research indicate that the foot print gradually improved in all three groups. There were significant statistical differences between end to end anastomosis with amniotic membrane coverage (-53.75+/-19.75) and simple end to end anastomosis (-79.68/+-11.79) groups in the 12th week (P=0.017). The best electrophysiological result was in the end to end anastomosis with amniotic coverage group. The electrophysiological results indicate that there are significant statistical differences between end to end anastomosis with amniotic membrane coverage and end to end anastomosis with vein conduit coverage groups (P=0.044). The histological results showed that the most axonal count were in the end to end anastomosis with amniotic membrane coverage group. The axonal count indicate that there were significant statistical differences between end to end anastomosis with amniotic membrane coverage and end to end anastomosis with vein conduit coverage groups (P=0.041). Conclusions: The result of this research, in line with results from similar studies, confirms a significant and beneficially effective Amniotic membrane on nerve grafts as compared with other grafts.
Article KeyWords:
Nerve Graft, Amniotic Membrane, Electromyography
Article Authors:

	دکتر سید کمال فروتن	First Author
	دکتر ابوالفضل عبد یزدانی	Other Author	dryazdanimd@yahoo.com
	دکتر حسین اکبری	Other Author
	دکتر محمد جواد فاطمـی	Other Author
	دکتـر میر سپهر پـدرام	Other Author
	دکتـر سید جابر مـوسوی	Other Author