Effects of Amniotic Fluid in Prevention of Abdominal Adhesion after Laparatomy in Rat Models

Nourian, S.E; Ghoraishi, M.; Goharian, V.; Sehhat, S.

Effects of Amniotic Fluid in Prevention of Abdominal Adhesion after Laparatomy in Rat Models

Document Type : Original Article

Authors

S.E Nourian ¹

M. Ghoraishi ²

V. Goharian ³

S. Sehhat ⁴

¹ Assistant Professor of General Surgery, Isfahan University of Medical Sciences and Health Services, Al-Zahra Hospital, Isfahan, Iran

² Resident of General Surgery, Isfahan University of Medical Sciences and Health Services, Al-Zahra Hospital, Isfahan, Iran

³ Resident of Thoracic Surgery, Isfahan University of Medical Sciences and Health Services, Al-Zahra Hospital, Isfahan, Iran

⁴ Professor of Surgery, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran

Abstract

Introduction & Objective: Many studies were done and designed to determine the pathophysiology
and to find a way to reduce the formation of adhesion bands after laparatomy. The purpose of our study was
to determine the effect of aminiotic fluid in prevention of post laparatomy adhesion formation in rat models.
Materials & Methods: Our study was experimental and laboratory. Thirty adult male rat were used in
this study, divided in to two equal groups randomly.
We opened the abdominal wall, then 1 * 1 cm peritoneal layer was excised from the left abdominal wall
and 10 longitudinal incision of 2 to 3 cm in length were made on the right parietal peritoneum after that we
filled the abdomen of the rats of group I (case group) with 5 cc of amniotic fluid and filled the abdominal of
the rats group II (control group) with 5 cc normal saline. Second laparatomy carried out 14 day after the first
laparatomy and abdominal adhesion were scored in order to Ahmet Canbaz scale and analyzed with Mann –
withney test; P< 0.05 was significant.
Results: After analysis we saw a significant difference between two group (P=0.008). The average of
adhesion score were 1.13 +1.3 in group I and 2.5 + 1.18 in group II (P<0.05).
Conclusions: Our study showed that Aminiotic fluid is capable to reduce the formation of adhesion bands
after laparatomy.

Keywords

Adhesion Band

Amniotic Fluid

Rat