Introduction & Objective: Staphylococcus Aureus (SA) is one of the most prevalent bacterial pathogens in human beings. Approximately 20% of healthy persons are persistent carriers and 60% are intermittent carriers of SA. Nasal cavity is one of the important sites of its colonization. The aim of this study was the study of the frequency of staphylococcus areus nasal carriers in the surgical staff of Hamadan Educational Hospitals, and it's antimicrobial susceptibility pattern. Materials & Methods: In a case-control study, 246 persons (123 persons from surgical staff of Hamadan Educational Hospitals and 123 persons from the healthy personnel of Hamadan university) entered in our study. A nasal soap was prepared from each patient for SA isolation and its antibiotical susceptibility were determined with antibiogram. Data were analyzed with the SPSS. Results: 17 persons (13.8%) of the study group and 14 persons (11.4%) of the control group had positive cultures for SA. There was no meaningful statistical difference in SA prevalence (P: 0.351). In the study group, there was no meaningful statistical difference between SA prevalence with ward, occupation and occupation time (p>0.05). In both groups all cases were resistant to penicillin and were sensitive to Vancomycin. In the case group all subjects were resistant to Clindamycin. In control group all subjects were senstive to cephazolin and cotrimoxazole. 59.2% of the cases and 35.7% of the controls were MRSA. Conclusions: Although in present the study the prevalence rate of nasal carriers of staphylococcus aureus was higher in medical personnel than healthy people, but the difference is not statistically significant. In this study, the number of carriers of medical personnel is less than reports from other countries.