Introduction & Objective: Adhesion formation after abdominal surgery is a major cause of postoperative bowel obstruction, infertility, and chronic abdominal pain. In this study, we evaluated the effect of normal saline on postoperative adhesion formation in a rat model. Materials & Methods: A total of 30 Wistar female rats were randomly divided in 3 groups involving Normal saline (NS) group, Control group and a group of normal animals (not include in our study). Under general anesthesia, and following a 3cm midline incision, the anterior surface of serous membrane in the rat was scraped slightly. One ml of Normal saline was administrated intraperitoneally in the NS group. The duration from opening to the beginning of the closure was 1 min. In the control group, no medication was administered, and only cecum was exposed to air for 1 min. Fourteen days after the surgery, a re-laparotomy was performed for macroscopic and pathological assessment and then all of them were destroyed. A comparison of the groups was carried out using the non-parametric Kruskal-Wallis test, followed by Mann-Whitney U statistics, to detect the statistically significant differences among the groups. Analysis was performed using SPSS 13. P-value ≤ 0.05 was considered significant. Results: The adhesion grade and extent of the normal saline group was lower than those of control group in macroscopic assessment (P