Introduction & Objective: Antimicrobial resistance is a major problem that imposes high costs on burn patients. Therefore, selection of newer methods in order to deal with complications and side effects such as infection or delay in wound healing is needed. The aim of this study was to compare Silver serum levels and acute toxic effects of Nano-silver on histopathology of organs (lungs, liver, kidney, brain) in two types of AgiCoat and Acticoat dressings on second-degree deep burn in rat. Materials & Methods: This is an experimental study conducted at animal laboratory of Hazrat Fatima Hospital in 1394. We divided 24 Sprague-Dawley male rats weighing 300 to 350 randomly into two groups. After burning dorsal skin of rats in the first and the second group, their dressings were changed every three days with AgiCoat and Acticoat respectively. After 14 days, we sent blood samples taken from heart, liver, kidneys, lungs and brain and a sample from dorsal skin of the rat to the lab in order to do histopathological examinations. Then the data were analyzed using by SPSS software, version 20 and one-sample, independent-sample test and ANOVA. Results: The results of this study showed that the level of silver serum per Microgram per Liter in the Agicoat group was 17.644±20.371 and in the Acticoat group was 21.800±12.199 and was a significant difference (AgiCoat = 0.017) (Acticoat = P < 0.001). However, in terms of silver serum levels in two groups, there was no significant difference (P = 0.551). Examination of The relationship between the level of silver serum and histopathological changes in liver showed that hepatotoxicity of Agicoat compared to Acticoat was higher and the different was significant (P = 0.002). Conclusions: These dressings can cause toxicity and liver pathology. Although it does not seem only dressings cause this problem. Other factors such as weight, host resistance, immune system and etc should be considered important. Since the increase in blood silver would lead to an excessive injury, we recommend further studies on these dressings and similar products.