Introduction & Objective: About a quarter of patients admitted to emergency departments complain of acute abdominal pain. Based on this, many surgeons believe that in relieving the pain of patients with acute abdominal pain, they have intervened with the results of clinical examinations and the possible final decision, and for this reason, it is necessary to use painkillers in these patients until a definitive diagnosis is reached. Appropriate treatment plan is delayed. Considering the high prevalence of patients with non-traumatic abdominal pain, the aim of this study was to investigate the effect of Apotel, morphine and ketorolac on the diagnostic process of symptoms in patients with acute non-traumatic abdominal pain. Materials & Methods: This study was carried out in a cross-sectional analytical manner. The research population consisted of patients with non-traumatic abdominal pain referred to Shahid Beheshti Hospital in Qom between 2018 and 2021. Demographic information of patients such as age, sex, etc., as well as other information required for the study were collected and compared in three groups of patients receiving Apotel, ketorolac and morphine. Finally, all information was entered into SPSS software version 22 and statistically analyzed with ANOVA and chi square tests. A significance level of less than 0.05 was considered. Results: A total of 150 patients were examined. The average age of the patients was 35.49 years with a standard deviation of ±17.14. 80 (53.3%) were male and 70 (46.7%) were female. No statistically significant difference was found between systolic blood pressure, heart rate, tenderness, rebound, guarding, between the groups receiving Apotel, ketorolac, and morphine (P > 0.05). But the diastolic blood pressure and the time to reach the diagnosis in the group receiving morphine were statistically significantly higher than the other two groups (P = 0.001). Conclusions: The results of the study showed that the injection of Apotel, morphine and ketorolac pain relievers not only has no significant effect on the diagnostic protocol of symptoms in patients with complaints of acute non-traumatic abdominal pain, but also relieves pain in patients.