Introduction & Objective: Obesity is one of the risk factors for heart diseases. Obese people are more likely to suffer from heart diseases such as angina pectoris, cardiac arrest and heart attack. Obesity can lead to increased blood pressure, high cholesterol, and insulin resistance, all of which are risk factors for heart disease. Based on this, the aim of the present research was the effect of aerobic exercise and consumption of pomegranate peel extract on the expression of CAT and SOD genes related to heart tissue in obese female rats, so that acceptable results can be achieved in this field. Materials & Methods: In this experimental study, 30 female Wistar rats with an average weight of 180 ± 20 were randomly divided into 5 groups: 1) healthy control, 2) obese control, 3) supplement, 4) exercise and 5) exercise supplement. Were divided Aerobic training program reached %50 VO2 max in the first week and %65 VO2 max in the last week. Pomegranate peel extract was prepared in liquid form and dissolved in distilled water and fed to rats by gavage at a dose of 60 mg per kilogram of body weight. Results: Aerobic exercise and pomegranate peel extract separately had a significant effect on CAT gene expression in heart tissue. The interaction of aerobic exercise and pomegranate peel extract also had a significant effect on CAT gene expression. Aerobic exercise alone did not significantly change CAT gene expression compared to the control group, while receiving pomegranate peel extract caused a significant change in CAT gene expression. Also, aerobic exercise and pomegranate peel extract, separately and in combination, had a significant effect on SOD gene expression in heart tissue. The interaction of aerobic exercise and pomegranate peel extract also had a significant effect on SOD gene expression in heart tissue. These results show that both interventions separately and in combination with each other have a significant effect on SOD gene expression in heart tissue. The results of Turkey’s follow-up test showed that SOD gene expression in the pomegranate peel extract group was significantly lower than the healthy control group. Conclusions: The results of the first hypothesis that a period of aerobic training and pomegranate peel extract have no significant effect on CAT gene expression in the heart tissue of obese female rats are not confirmed. Also, the results of the second hypothesis that a period of aerobic exercise and pomegranate peel extract have no significant effect on SOD gene expression in the heart tissue of obese female rats are not confirmed. Based on these results, it can be said that a period of aerobic exercise and pomegranate peel extract is effective on CAT and SOD gene expression in the heart tissue of obese female rats.