Introduction & Objective: Job satisfaction can be a significant and influential factor in the marital satisfaction. On the other hand, marital satisfaction is one of the most important factors affecting family functioning. Therefore, the present study aimed to determine the relationship between job satisfaction and the marital satisfaction among nurses working in operating rooms of educational therapeutic centers affiliated with Golestan University of Medical Sciences. Materials & Methods: This study employed a descriptive-analytical correlational design using a survey method. The participants included 79 nurses with a bachelor's degree or higher working in operating rooms of educational therapeutic centers affiliated with Golestan University of Medical Sciences. Data were collected using a demographic information form, the Loutanz Job Satisfaction Questionnaire (2007), and the Enrich Marital Satisfaction Questionnaire (1993). The collected data were analyzed using SPSS version 20 software, employing descriptive statistics, standard deviation, and Spearman correlation coefficient. Results: 57 women (72.2%) and 22 men (8.8%) participated in the study. The standard and deviation of marital quality is 17.7±0.2 and job satisfaction is 18.2±2.6, and no linear relationship between job satisfaction and marital relationship quality was observed in the group of men (P < 0.05, rs < 0.000). While in women, a linear relationship was observed between job satisfaction and the Marital Satisfaction, with the intensity of this relationship being very weak and in the opposite direction (P = 0.0001, rs = -0.054). Conclusions: The results of the present study indicate that gender has a weak association with job satisfaction and the Marital Satisfaction, which requires further investigation with a larger sample size. It is suggested that married working women attend classes in the form of retraining to improve family well-being.