Introduction & Objective: Gallbladder cancer is one of the gastrointestinal malignancies with a poor prognosis in Iran and other parts of the world. Although various studies have been conducted in Iran on its prevalence and risk factors, a comprehensive study that examines clinical and paraclinical findings in a large sample size has not been conducted. Therefore, this study was conducted to investigate the clinical and laboratory findings of patients with gallbladder cancer in a 10-year period. Materials & Methods: This retrospective study was conducted on 1856 patients who were referred to Imam Khomeini Hospital in Tehran and underwent open cholecystectomy from 1990 to 2000. First, according to the pathology report, the patients who were given a definitive diagnosis of gallbladder cancer were included in the study. Then clinical, laboratory, and imaging data were recorded using medical files records. Results: In this study, 32 patients with a definitive diagnosis of gallbladder cancer were enrolled. Most patients were female (18 patients, 56.2%) and the most common age range was 50 to 59 years (57.3±4.1 years). The right upper quadrant pain and loss of appetite were reported as the most (27 patients, 84.4%) and the least (2 patients, 6.2%) main complaints, respectively. Increased alkaline phosphatase and dilation of bile ducts were seen as the most common paraclinical findings in the studied patients. Conclusions: Gallbladder cancer is one of the invasive malignancies of the gastrointestinal tract in the Iranian population, which is more common among women and in the sixth decade of life. Abdominal pain and increased liver enzymes and alkaline phosphatase were seen as the most common clinical and paraclinical findings along with dilation of the bile ducts in the ultrasound examination among the patients.