Introduction & Objective: Evaluation of losing albumin and total protein in acute burning patient’s plasma important on prognosis on burning side effects. The aim of this study is the detection of total protein and albumin in acute burn patient's serum. Materials & Methods: In one cross-sectional study during12 month on 1396-1397 with simple method, 130 patients with age greater than 15 years old and the burning surface of 10-70% in Motahharie burning hospital was evaluated to serum total protein, albumin, BUN, and level of albumin to creatinine, data analysis was done with SPSS and soft ware version, 23 and for quantified subjects t-test with means and standard deviation and for quantified subjects chi-square-test with variation ± 0.05. Results: Mean level of serum's albumin, total protein, BUN, albumin to creatinine (ACR) in acute burning patients was respectively: 3.36±1.12, 5.14±0.46, 24.44±5.80, 0.89±0.14, 3.36±1.24 mg/dl. Inverse correlation to the percent of burn with serum albumin level (correlation coefficient equal -0.154) and total protein of serum (correlation coefficient equal -0.546, with) and ACR (correlation coefficient is -0.192. In addition, a direct Correlation coefficient on the percent of burn and BUN were seen 0.336 mg/dl .For allabove, P = 0.002. Conclusions: Hypoalbuminemia and Hypoproteinemia in acute burning patients is noticeable and direct relation with severity and surface of burning and two this injury were seen.